Evidence for differences in sterol biosynthesis and derivatization in sorghum

Heupel, R.C. and Nes, W.D. (1984) Evidence for differences in sterol biosynthesis and derivatization in sorghum. Journal of Natural Products, 47 (2). pp. 292-299.

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Abstract

The 24-desalkyl- and 24-alkylsterols synthesized by leaves of flowering sorghum plants were identified by a combination of GLC, HPLC, MS and NMR. Leaves from plants in the same developmental stage were treated with 2-14/C-acetate, and the radioactivity was associated with individual components of the 4,4-desmethyl sterol fraction determined by a combination of HPLC-radioactivity counting and GLC/MS. The specific activities of sorghum leaf sterols are in the following decreasing order: cholesterol (1036 dpm/µg)>24α-ethylcholesterol (416 dpm/µg)>24α-methylcholesterol (389 dpm/µg)>24α-ethylcholesta-5,22-dienol (39 dpm/µg). While all 4 of these sterols were detected by GLC as components of steryl glycosides and steryl esters, only the 24-alkysterols were present as 14C-labelled steryl glycosides. The differential regulation of sterol biosynthesis and derivatization is discussed in terms of differences in their functions

Item Type: Article
Author Affiliation: Plant Physiology and Chemistry Research Unit, WRRC-ARS/USDA, Department of Agriculture, Berkeley, CA 94710, USA
Subjects: Plant Physiology and Biochemistry > Plant Physiology
Plant Physiology and Biochemistry > Biochemistry
Divisions: Sorghum
Depositing User: Ms K Syamalamba
Date Deposited: 14 May 2012 05:18
Last Modified: 14 May 2012 05:18
Official URL: http://dx.doi.org/10.1021/np50032a010
URI: http://eprints.icrisat.ac.in/id/eprint/5283

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