Heupel, R.C. and Nes, W.D. (1984) Evidence for differences in sterol biosynthesis and derivatization in sorghum. Journal of Natural Products, 47 (2). pp. 292-299.
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Abstract
The 24-desalkyl- and 24-alkylsterols synthesized by leaves of flowering sorghum plants were identified by a combination of GLC, HPLC, MS and NMR. Leaves from plants in the same developmental stage were treated with 2-14/C-acetate, and the radioactivity was associated with individual components of the 4,4-desmethyl sterol fraction determined by a combination of HPLC-radioactivity counting and GLC/MS. The specific activities of sorghum leaf sterols are in the following decreasing order: cholesterol (1036 dpm/µg)>24α-ethylcholesterol (416 dpm/µg)>24α-methylcholesterol (389 dpm/µg)>24α-ethylcholesta-5,22-dienol (39 dpm/µg). While all 4 of these sterols were detected by GLC as components of steryl glycosides and steryl esters, only the 24-alkysterols were present as 14C-labelled steryl glycosides. The differential regulation of sterol biosynthesis and derivatization is discussed in terms of differences in their functions
Item Type: | Article |
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Author Affiliation: | Plant Physiology and Chemistry Research Unit, WRRC-ARS/USDA, Department of Agriculture, Berkeley, CA 94710, USA |
Subjects: | Plant Physiology and Biochemistry > Plant Physiology Plant Physiology and Biochemistry > Biochemistry |
Divisions: | Sorghum |
Depositing User: | Ms K Syamalamba |
Date Deposited: | 14 May 2012 05:18 |
Last Modified: | 14 May 2012 05:18 |
Official URL: | http://dx.doi.org/10.1021/np50032a010 |
URI: | http://eprints.icrisat.ac.in/id/eprint/5283 |
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