Perez-Carrillo, E. and Serna-Saldivar, S.O. and Chuck-Hernandez, C. and Cortes-Callejas, M.L. (2012) Addition of protease during starch liquefaction affects free amino nitrogen, fusel alcohols and ethanol production of fermented maize and whole and decorticated sorghum mashes. Biochemical Engineering Journal. pp. 1-27.
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Abstract
The aim of this research was to study the effect of the dual treatment of sorghum decortication and protease addition during liquefaction with α-amylase, on the concentration of free amino nitrogen (FAN), fusel alcohols and ethanol during yeast fermentation. A bifactorial experiment was designed to revise the differences among grains (maize, whole and 9.7% decorticated sorghum) and the effectiveness of protease addition during liquefaction. The decorticated sorghum was more susceptible to protein hydrolysis compared to the whole kernel sorghum due to its lower fiber content. The protease improved the levels of FAN approximately to 60 and 30% in the maize and sorghum mashes, respectively. The maize mash contained the highest amount of FAN followed by the decorticated and whole sorghum mashes. The protease treatment improved the fusel alcohol concentration in both sorghum beers and did not affect ethanol concentration in the maize mash. Both sorghum decortication and protease addition during liquefaction are therefore recommended treatments to obtain mashes with a higher FAN level. The decorticated protease-treated sorghum mashes yielded higher amounts of ethanol compared to the maize treatments and produced the maximum ethanol after only 20 hours of fermentation. This research proved that a positive synergistic effect on FAN concentration, fusel alcohols and bioethanol yields can be achieved through the proposed proceeding of sorghum decortication and protease addition.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Sorghum; decortication; protease; free amino nitrogen; fusel alcohols; bioethanol |
| Author Affiliation: | Centro de Biotecnología, Tecnológico de Monterrey, Av. Eugenio Garza Sada 2501 Sur, CP 64849, Monterrey, N.L. México |
| Subjects: | Plant Protection Crop Improvement Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Maize Sorghum |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 08 May 2012 05:46 |
| Last Modified: | 08 May 2012 05:46 |
| Official URL: | http://dx.doi.org/10.1016/j.bej.2012.04.010 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/5153 |
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