Cunillera, N. and Boronat, A. and Ferrer, A. (2000) Spatial and temporal patterns of GUS expression directed by 50 regions of the Arabidopsis thaliana farnesyl diphosphate synthase genes FPS1 and FPS2. Plant Molecular Biology, 44. pp. 747-758.
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Abstract
Farnesyl diphosphate synthase (FPS), the enzyme that catalyses the synthesis of farnesyl diphosphate (FPP) from isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), is considered a regulatory enzyme of plant isoprenoid biosynthesis. The promoter regions of the FPS1 and FPS2 genes controlling the expression of isoforms FPS1S and FPS2, respectively, were fused to the �-glucuronidase (GUS) reporter gene and introduced into Arabidopsis thaliana plants. The FPS1S:GUS gene is widely expressed in all plant tissues throughout development, thus supporting a role for FPS1S in the synthesis of isoprenoids serving basic plant cell functions. In contrast, the FPS2:GUS gene shows a pattern of expression restricted to specific organs at particular stages of development. The highest levels of GUS activity are detected in flowers, especially in pollen grains, from the early stages of flower development. After pollination, much lower levels of GUS activity are detected in the rest of floral organs, with the exception of the ovary valves, which remain unstained throughout flower development. GUS activity is also detected in developing and mature seeds. In roots, GUS expression is primarily detected at sites of lateral root initiation and in junctions between primary and secondary roots. No GUS activity is detected in root apical meristems. GUS expression is also observed in junctions between primary and secondary stems. Overall, the pattern of expression of FPS2:GUS suggests a role for FPS2 in the synthesis of particular isoprenoids with specialized functions. Functional FPS2 gene promoter deletion analysis in transfected protoplasts and transgenic A. thaliana plants indicate that all the cis-acting elements required to establish the full pattern of expression of the FPS2 gene are contained in a short region extending from positions
| Item Type: | Article |
|---|---|
| Additional Information: | We thank the Serveis Científico-Tècnics de la Universitat de Barcelona for technical assistance. We also thank Jordi Capella for his help and advice in preparing the colour photographs. This work was funded by Grants BIO4-962077 from the EC Biotechnology Programme, PB96-0176 from the Dirección General de Investigación Científica y Técnica del Ministerio de Educación y Cultura, and 1997SGR-00088 from the Comissió Interdepartamental de Recerca i Innovació Tecnològica de la Generalitat de Catalunya. |
| Uncontrolled Keywords: | Arabidopsis thaliana, �-glucuronidase (GUS) reporter gene, farnesyl diphosphate synthase, gene expression, isoprenoids, transgenic plants |
| Author Affiliation: | 1Departament de Bioqu´ımica i Biologia Molecular, Facultat de Farm`acia, Universitat de Barcelona, Av. Diagonal 643, 08028-Barcelona |
| Subjects: | Crop Improvement > Genetics/Genomics |
| Divisions: | General |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 27 Apr 2012 08:14 |
| Last Modified: | 27 Apr 2012 08:14 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/4985 |
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