Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa

Achaleke, J and Martin, T and Ghogomu, R T and et al, . (2009) Esterase-mediated resistance to pyrethroids in field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) from Central Africa. Pest Management Science, 65 (10). pp. 1147-1154.

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Abstract

BACKGROUND: Evolution of pyrethroid resistance in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) threatens continued cotton production in Central Africa. Dose–response bioassays were conducted on area-wide collection of bollworm populations from major host plants, while biochemical techniques were used to evaluate basic mechanisms underlying resistance. RESULTS: Pyrethroid resistance is primarily associated with detoxification by enhanced esterase activity. High resistance to cypermethrin (RF = 67–1771), cross-resistance to deltamethrin (RF = 60–2972) and lack of cross-resistance to the non-ester pyrethroid etofenprox (RF = 2–10) were observed among H. armigera field populations and laboratory-selected strains. Enzymatic assays showed that esterase activity, but not oxidase content or glutathione-S-transferase activity, was positively correlated with resistance to cypermethrin. Pretreatment with piperonyl butoxide (PBO) resulted in significant synergism with cypermethrin in 6/10 field populations, but not in the laboratory-selected strain, indicating that additional mechanisms such as mixed-function oxidase (MFO) may be involved in field resistance. The absence of cross-resistance to DDT ruled out a possible target-site modification. CONCLUSION: Knowledge of the mechanisms involved in pyrethroid resistance and the lack of cross-resistance to spinosad and indoxacarb is a key to devising new resistance management strategies aimed at restoring the efficacy of pyrethroid-based programmes. Copyright © 2009 Society of Chemical Industry

Item Type: Article
Additional Information: The authors express their sincere gratitude to PRASAC/ARDESAC, IFS Grant No. C/4067-1, and the National Cotton Development Corporation (SODECOTON) for their financial support. Special thanks go to O. Njikam and CREC-IRD for technical assistance. The authors are particularly grateful toDrGrahamDMoores,Dr Yidong Wu and the two anonymous reviewers for comments and helpful suggestions on an earlier version of the manuscript.
Uncontrolled Keywords: insecticide resistance;esterase;synergist;cotton;pyrethroids;Cameroon;Helicoverpa armigera
Author Affiliation: IRAD, PRASAC-ARDESAC, Cotton Programme, Garoua, Cameroon
Subjects: Plant Protection
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 19 Apr 2012 04:22
Last Modified: 19 Apr 2012 04:22
Official URL: http://dx.doi.org/10.1002/ps.1807
URI: http://eprints.icrisat.ac.in/id/eprint/4837

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