Liu, F and Xu, Z and Chang, J and et al, .
(2008)
Resistance Allele Frequency to Bt Cotton in Field Populations of Helicoverpa armigera (Lepidoptera: Noctuidae) in China.
Journal of Economic Entomology, 101 (3).
pp. 933-943.
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Abstract
Resistance evolution in target insects to Bacillus thurningiensis (Bt) cotton, Gossypium
hirsutum L., is a main threat to Bt cotton technology. An increasing trend of population density of
Helicoverpa armigera (Hu¨ bner) (Lepidoptera: Noctuidae) has been observed since 2001 in Qiuxian
County (Hebei, China), where Bt cotton has been planted dominantly since 1998. This region was
selected in 2006 and 2007 for estimating frequency of gene alleles conferring resistance to Bt cotton
by screening the F1 progeny from single-pair cross between Þeld-collected male and laboratory female
of the Bt-resistant strain of H. armigera (F1 screen). F1 offspring from each single-pair line were
screened for resistance alleles based on larval growth, development, and survival on Bt cotton leaves
for 5 d. Two-year results indicated that �20% of Þeld-collected males carried resistance alleles. The
conservative estimate of the resistance allele frequency was 0.094 (95% CI, 0.044Ð0.145) for 2006 and
0.107 (95% CI, 0.055Ð0.159) for 2007. This is the Þrst report of resistance allele frequency increase to
such a high level in the Þeld in China. Long-term adoption of Bt sprays, dominant planting of
single-toxinÐproducing Bt cotton, and lack of conventional cotton refuge system might accelerate the
resistance evolution in the region.
| Item Type: |
Article
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| Additional Information: |
We are grateful to Qiuxian County Plant Protection Station
for providing facilities and Þeld data of bollworm occurrence.
Special thanks to Fred Gould (North Carolina
State University, Raleigh, NC) for valuable advices and the
help with the data analysis and to Andrew Li (USDAÐARS,
Kerrville, TX) and Lingxiao Zhang (DREC, Mississippi State
University, Stoneville, MS) for valuable comments and suggestions
for improving an earlier version of this manuscript.
This research was supported by the Special Funding of Transgenic
Plant Study and Its Industrialization Opening up and
Developing (J00-C-002) and National ScientiÞc Research
Fund (30270889). |
| Uncontrolled Keywords: |
Helicoverpa armigera, Bt cotton, resistance detection, F1 screen, resistance management |
| Author Affiliation: |
Department of Pesticide Science, College of Plant Protection,
Nanjing Agricultural University, Nanjing, 210095, China. |
| Subjects: |
Plant Protection |
| Divisions: |
General |
| Depositing User: |
Mr Siva Shankar
|
| Date Deposited: |
16 Apr 2012 04:38 |
| Last Modified: |
16 Apr 2012 04:38 |
| URI: |
http://eprints.icrisat.ac.in/id/eprint/4719 |
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