Inhibition of lipoxygenase-1 by tetrahydrocurcumin

Sneharani, A H and Singh, S A and Srinivas, P and et al, . (2011) Inhibition of lipoxygenase-1 by tetrahydrocurcumin. European Food Research and Technology, 233 (4). pp. 561-568.

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Abstract

Tetrahydrocurcumin (THC)—the reduced form of curcumin—is more hydrophilic than its parent compound. It possesses higher stability in aqueous medium compared to curcumin. Lipoxygenase (LOX) enzyme is implicated in inflammatory conditions. THC was investigated for the inhibition of soy LOX-1. THC-inhibited LOX-1 with an IC50 value of 44.6 ± 0.6 μM. Kinetics of inhibition revealed mixed linear type with a K i value of 46 μM. THC-inhibited LOX-1 by preventing the activation of LOX-1 as evidenced from spectroscopic studies. Molecular docking simulations suggested the binding of THC near the iron cofactor. This study helps in further understanding of the anti-inflammatory property of curcumin derivatives and provides valuable leads as an alternative of curcumin with better solubility and stability at physiological pH.

Item Type: Article
Additional Information: Authors thank Dr. V. Prakash, Director, CFTRI, for providing the facilities. A.H.S. is grateful for the fellowship awarded by the University Grants Commission, Government of India.
Uncontrolled Keywords: Tetrahydrocurcumin, Lipoxygenase, Redox inhibition, Spectroscopic measurements, Stability
Author Affiliation: 1.Department of Protein Chemistry and Technology, Central Food Technological Research Institute (A Constituent Laboratory of the Council of Scientific and Industrial Research), Mysore, 570020 India
Subjects: Crop Improvement
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 13 Apr 2012 08:32
Last Modified: 13 Apr 2012 08:32
Official URL: http://dx.doi.org/10.1007/s00217-011-1541-6
URI: http://eprints.icrisat.ac.in/id/eprint/4712

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