Wecksler, A T and Garcia, N K and Holman, T R (2009) Substrate specificity effects of lipoxygenase products and inhibitors on soybean lipoxygenase-1. Bioorganic & Medicinal Chemistry, 17 (18). pp. 6534-6539.
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Abstract
Recently, it has been shown that lipoxygenase (LO) products affect the substrate specificity of human 15- LO. In the current paper, we demonstrate that soybean LO-1 (sLO-1) is not affected by its own products, however, inhibitors which bind the allosteric site, oleyl sulfate (OS) and palmitoleyl sulfate (PS), not only lower catalytic activity, but also change the substrate specificity, by increasing the arachidonic acid (AA)/ linoleic acid (LA) ratio to 4.8 and 4.0, respectively. The fact that LO inhibitors can lower activity and also change the LO product ratio is a new concept in lipoxygenase inhibition, where the goal is to not only reduce the catalytic activity but also alter substrate selectivity towards a physiologically beneficial product.
| Item Type: | Article |
|---|---|
| Additional Information: | The authors acknowledge the Holman group members for helpful discussions and proof reading the manuscript. |
| Uncontrolled Keywords: | Soybean lipoxygenase-1, Allosteric inhibition, Substrate specificity |
| Author Affiliation: | Chemistry and Biochemistry Department, University of California, Santa Cruz, CA 95064, United States |
| Subjects: | Crop Improvement |
| Divisions: | Soyabean |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 11 Apr 2012 09:09 |
| Last Modified: | 11 Apr 2012 09:09 |
| Official URL: | http://dx.doi.org/10.1016/j.bmc.2009.08.005 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/4645 |
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