Cellular localization of dual positional specific maize lipoxygenase-1 in transgenic rice and calcium-mediated membrane association

Cho, K and Han, Y and Woo, J C (2011) Cellular localization of dual positional specific maize lipoxygenase-1 in transgenic rice and calcium-mediated membrane association. Plant Science, 181 (3). pp. 242-248.

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Abstract

The dual positional maize lipoxygenase-1 was introduced into rice and T2 transgenic plants were produced. Cellular location of maize lipoxygenase-1 in transgenic rice and effects of calcium ion on membrane association in vitro were analyzed. Localization study by confocal microscopic analysis indicated that the maize lipoxygenase-1 was localized in cytoplasm. Sucrose-density fractionation experiment and in vitro protein transport to chloroplast showed that the maize lipoxygenase-1 can be associated with chloroplast. Secondary structure alignment revealed putative calcium binding sites in the PLAT domain of maize lipoxygenase-1 and the association of the maize lipoxygenase-1 with membranes was mediated by calcium ion in vitro. Our results provide evidences for calcium-mediated translocation of dual positional LOX without chloroplast targeting sequence from cytoplasm to chloroplast in plants for the first time.

Item Type: Article
Additional Information: This work was supported by Korea Research Foundation (2008-313-F00020) to O. Han.
Uncontrolled Keywords: Lipoxygenase; Dual positional specificity; Translocation; Calcium; Maize; Rice
Author Affiliation: Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea
Subjects: Crop Improvement
Divisions: Maize
Depositing User: Mr Siva Shankar
Date Deposited: 11 Apr 2012 08:58
Last Modified: 11 Apr 2012 08:58
Official URL: http://dx.doi.org/10.1016/j.plantsci.2011.05.016
URI: http://eprints.icrisat.ac.in/id/eprint/4643

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