Alfaro-Fernández, A. and Ali, M.A. and Abdelraheem, F.M. (2012) Molecular identification of 16SrII-D subgroup phytoplasmas associated with chickpea and faba bean in Sudan. European Journal of Plant Pathology. pp. 1-5.
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Abstract
In January 2011, symptomatic chickpea and faba bean plants were observed in fields located in the Gezira state (Sudan). Faba bean plants showed yellowing and stunting, whereas chickpea plants presented yellowing, reddening and little leaves. The disease etiology was investigated using nested polymerase chain reaction (PCR) with phytoplasma-specific primers which amplify a fragment of the 16S rRNA gene. Sequencing and restriction fragment length polymorphism (RFLP) analyses revealed that the tested phytoplasmas belonged to the group 16SrII. Phylogenetic analyses of the 16S rRNA gene of the obtained sequences indicated that the chickpea and faba bean phytoplasmas from Sudan were more closely related to the phytoplasmas subgroup 16SrII-D. To our knowledge, this is the first report of phytoplasmas from the group 16SrII-D infecting chickpea in Sudan, and faba bean worldwide.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Cicer arietinum – Group 16SrII – PCR – RFLP – Vicea faba |
| Author Affiliation: | Grupo de Virología, Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia (IAM-UPV), Camino de Vera s/n., 46022 Valencia, Spain, Plant Pathology Center, Faculty of Agricultural Sciences, University of Gezira, P.O. Box 20, Wad Medani, Sudan |
| Subjects: | Crop Improvement |
| Divisions: | Chickpea Common Bean |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 08 Apr 2012 05:18 |
| Last Modified: | 08 Apr 2012 05:19 |
| Official URL: | http://dx.doi.org/10.1007/s10658-012-9975-7 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/4551 |
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