Efficient in vitro regeneration in Pigeonpea from cotyledonary node explants

Kaur, A. and Devi, R. and Dev, A. (2012) Efficient in vitro regeneration in Pigeonpea from cotyledonary node explants. Journal of Cell and Tissue Research, 12 (1). pp. 3075-3080.

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Abstract

A reproducible method of direct and multiple shoot induction has been developed in pigeonpea using variety AL 201. Cotyledonary nodal explants excised from 5 and 8 day old in vitro raised seedlings were cultured on basal MS, MS + 3 mg/lBAP + 1mg/lKin andMS + 3mg/ l BAP + 1 mg/l Kin + 0.2% w/v charcoal media. Data were recorded on per cent responding cultures, average number of shoots and shoot length per explant. Eight-day-old explants responded better than 5 day old explants for multiple shoot induction. The average number of shoots and shoot length per explant were highest (3.19 and 11.27 cm, respectively) on medium containing charcoal. Shoot elongation and rooting in 80% of shoots was obtained on charcoal medium itself. After hardening, the plantletswere transferred to soil in poly bags and kept in the glasshouse. Thus this direct plant regeneration system so developed can be used as a base line for genetic transformation of pigeonpea

Item Type: Article
Author Affiliation: School ofAgricultural Biotechnology, PunjabAgricultural University, Ludhiana
Subjects: Crop Improvement
Divisions: Pigeonpea
Depositing User: Mr. SanatKumar Behera
Date Deposited: 03 Apr 2012 14:39
Last Modified: 03 Apr 2012 14:39
Official URL: http://www.tcrjournals.com/tr_currentfullpaper.php...
URI: http://eprints.icrisat.ac.in/id/eprint/4505

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