Radioimmunoassay for the determination of free and conjugated abscisic acid

Weiler, E W (1979) Radioimmunoassay for the determination of free and conjugated abscisic acid. Planta, 144 (3). pp. 255-263.

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Abstract

The characterization and application of a radioimmunoassay specific for free and conjugated abscisic acid (ABA) is reported. The antibodies produced against a bovine serum albumin-(±)-ABA conjugate have a high affinity for ABA (Ka=1.3x109l mol-1). Trans, trans-ABA and related compounds, such as xanthoxin, phaseic acid, dihydrophaseic acid, vomifoliol or violaxanthin do not interfere with the assay. The detection limit of this method is 0.25x10-12 mol ABA, the measuring range extends to 20x10-12 mol, and average recoveries are 103%. Because of the high specificity of this immunoassay, no extract purification steps are required prior to analysis. Several hundred plants can be analyzed per day in a semi-automatic assay performance. ABA has been detected in all higher plant families examined, but was absent in the blue-green alga, Spirulina platensis, the liverwort Marchantia polymorpha, and two species of fungi.

Item Type: Article
Additional Information: Phaselc acid was a gIft from Prof Raschke East Lansing xanthoxm was from Dr Bottger, Hamburg, and the vomlfoThhoel was a gift from Dr Kapil, Lucknow Prof Kmdl, Marburg, provided lunulanc acid, and Dr Oklta, Sendal, provided compounds (X) and (XI) The excellent techmcal assistance of Mrs P Westekemper is also acknowledged, as well as the proof reading of the manuscript by Prof R Manse11 Tampa
Uncontrolled Keywords: AbscislC acid, Persea, Radioimmunoassay
Author Affiliation: Ruhr-Universität Bochum
Subjects: Crop Improvement > Plant Breeding
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 30 Mar 2012 09:42
Last Modified: 30 Mar 2012 09:42
Official URL: http://dx.doi.org/10.1007/BF00388767
URI: http://eprints.icrisat.ac.in/id/eprint/4411

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