Saijo, R. and Kosuge , T. (1978) The conversion of 3-deoxyarabinoheptulosonate 7-phosphate to 3-dehydroquinate by sorghum seedling preparations. Phytochemistry, 17 (2). 223-225 .
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Abstract
Partially-purified preparations from etiolated sorghum seedlings catalysed the conversion of DAHP to DHQ. The reaction catalysed by DHQ synthetase was stimulated by 0.1 mu M to 0.1 mM NAD in the presence of 0-0.5 mM Co2+. NADH at 1 mu M stimulated the reaction as much as 50% but became inhibitory at 100 mu M. Co2+ at 0.5 mM stimulated enzyme activity 3-fold; Mg2+, Mn2+, Cu2+, and Zn2+ were not stimulatory, EDTA at 5 mM inhibited the reaction 95% but its effects were reversed by equal concentrations of Co2+. Phenylalanine, tyrosine, tryptophan, t-cinnamate, several hydroxylated cinnamates, 3-dehydroshikimate, quinate and shikimate at 0.3 mM failed to affect enzyme activity, but slight inhibition occurred with DHQ and protocatechuic acid at 0.3 mM, inhibition being 14% and 22%, resp. DHQ synthetase activity was also detected in potato tuber tissue. Synthetase activity appeared to increase in response to injury of potato and sweet potato tuber tissues
| Item Type: | Article |
|---|---|
| Author Affiliation: | Department of Plant Pathology, California University, Davis, CA 95616, USA |
| Subjects: | Crop Improvement > Seed Technology |
| Divisions: | Sorghum |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 30 Mar 2012 09:31 |
| Last Modified: | 25 Apr 2012 04:45 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/4409 |
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