Lysine Estimation in Cereals by Near-Infrared Reflectance

Rubenthaler, G.L. and Bruinsma, B.L. (1978) Lysine Estimation in Cereals by Near-Infrared Reflectance. Crop Science, 18 (6). pp. 1039-1042.

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Abstract

The determination of lysine in cereals has been limited by methods which are slow and relatively expensive. Experimental data obtained with a standard near-infrared- reflectance (NIR) instrument — commercially available to determine protein, moisture, lipids, and other constituents of cereals and oil seeds — were highly correlated (r = 0.98) with lysine in some 77 samples of hard red (spring and winter) wheats (Triticum aestivum L.). Standard error (Sy•x) for the test was ±0.049 mg lysine/ 100 g protein. The method is rapid (1 min), relatively accurate, involves little sample preparation, and provides direct readout of lysine. The test is non-destructive and, therefore, the sample can be used for other quality tests. Minimum sample required is approximately 2 g and optimum is 6 to 8 g. Care in sample selection, preparation (grinding and filling the sample cell), and monitoring with a reference method is necessary for consistent meaningful results

Item Type: Article
Author Affiliation: Research food technoloeist in charge, Western Wheat Quality Laboratory, SEA-USDA, Pullman, WA 99164
Subjects: Crop Improvement
Divisions: Millet
Sorghum
Depositing User: Ms K Syamalamba
Date Deposited: 29 Mar 2012 10:23
Last Modified: 29 Mar 2012 10:23
Official URL: http://dx.doi.org/10.2135/cropsci1978.0011183X0108...
URI: http://eprints.icrisat.ac.in/id/eprint/4366

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