Simple sequence repeat-based diversity in elite pigeonpea genotypes for developing mapping populations to map resistance to Fusarium wilt and sterility mosaic disease

Saxena, R.K. and Saxena, K.B. and Kumar, R.V. and Hoisington, D.A. and Varshney, R.K. (2010) Simple sequence repeat-based diversity in elite pigeonpea genotypes for developing mapping populations to map resistance to Fusarium wilt and sterility mosaic disease. Plant Breeding, 129 (2). pp. 135-141.

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Abstract

In order to maximize polymorphism in the mapping populations for mapping loci for Fusarium wilt (FW) and sterility mosaic disease (SMD) resistance in pigeonpea, a set of 32 pigeonpea lines were screened for polymorphism with 30 microsatellite or simple sequence repeat markers. A total of 23 marker loci showed polymorphism with 2–4 alleles and the polymorphism information content for these markers ranged from 0.12 to 0.65 with an average of 0.43 per marker. High number of polymorphic markers, higher genetic dissimilarity coefficient and contrasting phenotypic data taken into consideration and five parental combinations were identified and crosses initiated for developing five genetically diverse mapping populations. Of these crosses, one cross segregates for FW resistance, two for SMD resistance and the remaining two crosses segregate for resistance to both FW and SMD. Development of mapping populations is in progress for mapping loci for resistance to FW and SMD in pigeonpea.

Item Type: Article
Author Affiliation: Centre of Excellence in Genomics (CEG), International Crops Research Institute for the Semi-Arid Tropics (ICRISAT),Patancheru, Greater Hyderabad- 502324, AP, India
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Divisions: Pigeonpea
Depositing User: Sandhya Gir
Date Deposited: 01 Nov 2010 20:05
Last Modified: 29 Dec 2010 20:53
Official URL: http://dx.doi.org/10.1111/j.1439-0523.2009.01698.x
URI: http://eprints.icrisat.ac.in/id/eprint/430

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