Tissue localization of polyphenol oxidase in Sorghum

Vaughn, K. C and Duke, S. O. (1981) Tissue localization of polyphenol oxidase in Sorghum. Protoplasma, 108 (3-4). pp. 319-327.

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Abstract

Plastidic polyphenol oxidase (PPO) was localized in various plastid types ofSorghum bicolor (L.) Moench using cytochemical and biochemical franctionation techniques. PPO was found to be present in the mesophyll plastids yet absent from the bundle sheath and guard cell plastids. Mechanical fractionation of mesophyll and bundle sheath plastids, with subsequent electrophoretic or spectrophotometric assay of the preparations, also indicated that PPO was absent from the bundle sheath but present in the mesophyll fraction. A developmental study revealed that, although all leaf plastids near the basal meristem were ultrastructurally similar, the mesophyll and bundle sheath plastids were already differentiated with respect to PPO activity.

Item Type: Article
Author Affiliation: Science, Weed Science Laboratory, USDASFA-AR, Stoneville, Missisippi
Subjects: Crop Improvement
Divisions: Sorghum
Depositing User: Library ICRISAT-InfoSAT
Date Deposited: 28 Mar 2012 06:46
Last Modified: 28 Mar 2012 06:46
Official URL: http://dx.doi.org/10.1007/BF02224426
URI: http://eprints.icrisat.ac.in/id/eprint/4261

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