Cytochemical Localization of Photosystem II Donor Sites

Vaughn, KC and Duke, SO (1981) Cytochemical Localization of Photosystem II Donor Sites. Histochemistry, 73 (3). pp. 363-369.

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Abstract

Tetramethylbenzidene, an artificial donor of electrons to the photosystem (PS) II reaction center, is oxidized to an osmiophilic polymer that allows for the localization of the donor site of PS II. Mesophyll chloroplasts of Sorghum bicolor (L.) Moench. contain 0.1-0.2 micron deposits only in the grana stacks and on the lumen side of the thylakoid. Agranal mature bundle sheath plastids (known to be devoid of PS II activity) show little deposition and the products appear randomly distributed along the lamellae. The cytochemical localization of PS II donor sites on the lumen side is consistent with flash photolysis and diaminobenzenesulfonic acid inactivation studies as well as the chemiosmotic theory for the generation of a proton gradient.

Item Type: Article
Author Affiliation: Southern weeds Science Laboratory, USDA-SEA AR PO-225 Stone-ville MS 38776 USA
Subjects: Plant Production > Farming Systems
Plant Physiology and Biochemistry > Biochemistry
Divisions: Millet
Depositing User: Library ICRISAT-InfoSAT
Date Deposited: 28 Mar 2012 08:31
Last Modified: 28 Mar 2012 08:31
URI: http://eprints.icrisat.ac.in/id/eprint/4238

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