Identification of quantitative trait loci for bruchid (Callosobruchus maculatus) resistance in black gram [Vigna mungo (L.) Hepper]

Souframanien, J. and Gupta, S.K. and Gopalakrishna, T. (2010) Identification of quantitative trait loci for bruchid (Callosobruchus maculatus) resistance in black gram [Vigna mungo (L.) Hepper]. Euphytica, 176 (3). pp. 349-356.

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Abstract

An inter-subspecific mapping population was generated by crossing V. mungo var. mungo (cv. TU 94-2, bruchid susceptible) and V. mungo var. silvestris (bruchid resistant). About 37.8% of the bruchid completed their lifecycle on seeds of V. mungo var. silvestris compared with 100% on the susceptible variety TU 94-2. The total developmental period of C. maculatus on Vigna mungo var. silvestris was considerably extended (88 days as compared with 34 days on TU 94-2). A genetic linkage map constructed using recombinant inbred lines (RILs) in F9 generation with 428 markers [86 random amplified polymorphic DNA (RAPD), 47 simple sequence repeat (SSR), 41 inter-SSR (ISSR), 254 amplified fragment length polymorphism (AFLP)] was used for QTL detection. One hundred four individuals were used for detection of QTLs associated with bruchid resistance. The RILs exhibited a high level of variation in percentage adult emergence (0–100%) and developmental period (0–105 days). Two QTLs, Cmrae1.1 and Cmrae1.2, were identified for percentage adult emergence, on linkage group (LG) 3 and 4, respectively. For developmental period, six QTLs were identified, with two QTLs (Cmrdp1.1 and Cmrdp1.2) on LG 1, three QTLs (Cmrdp1.3, Cmrdp1.4, and Cmrdp1.5) on LG 2, and one QTL (Cmrdp1.6) on LG 10.

Item Type: Article
Author Affiliation: Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India
Subjects: Crop Improvement > Biotechnology
Divisions: Other Crops
Depositing User: Sandhya Gir
Date Deposited: 01 Nov 2010 17:23
Last Modified: 29 Dec 2010 20:54
Official URL: http://dx.doi.org/10.1007/s10681-010-0210-3
URI: http://eprints.icrisat.ac.in/id/eprint/423

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