Hagerman, A.E. and Butler, L.G. (1980) Determination of protein in tannin-protein precipitates. Journal of Agricultural and Food Chemistry, 28 (5). pp. 944-947.
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Abstract
The amount of protein precipitated by tannin under a variety of conditions is measured by wing radioiodinated protein. The protein is mixed with purified tannin, finely ground plant tiseue, or an unpurified plant extract; the mixture is centrifuged to remove the insoluble tannin-protein complex. An aliquot of the supernatant layer is counted to calculate the amount of protein precipitated. Complex formation is dependent on the pH and solvent composition. Tannin specific activity ie wful for comparing tannin from varioue eources and for monitoring tannin purification. It is defined here as the ratio between the amount of protein precipitated and the amount of oxidizable material present
| Item Type: | Article |
|---|---|
| Author Affiliation: | Department of Biochemistry, Purdue University of West Lafayette, Indiana 47907 |
| Subjects: | Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 27 Mar 2012 05:23 |
| Last Modified: | 27 Mar 2012 05:39 |
| Official URL: | http://dx.doi.org/10.1021/jf60231a010 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/4207 |
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