Hagerman, A.E. and Butler, L.G. (1978) Protein precipitation method for the quantitative determination of tannins. Journal of Agricultural and Food Chemistry, 26 (4). pp. 809-812.
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Abstract
The tannin content of crude plant exts. or of purified prepns. was detd. by adding the sample to a std. soln. of protein, isolating the insol. tannin-protein complex, dissolving it in alk. soln., and measuring the absorbance at 510 nm after adding FeCl3. Plots of absorbance as a function of the amt. of tannin are linear for 0.20-1.0 mg tannic acid and partially purified sorghum tannins. Nontannin components of crude MeOH exts. of sorghum and cowpeas do not interfere with the assay. The results of the pptn. method are qual. similar to those obtained by vanillin assay. The pptn. assay can be used to study the effects of pH and other parameters on tannin-protein interactions
| Item Type: | Article |
|---|---|
| Author Affiliation: | Department of Biochemistry, Purdue University, West Lafayette, Ink. 47907 |
| Subjects: | Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 27 Mar 2012 04:55 |
| Last Modified: | 27 Mar 2012 04:55 |
| Official URL: | http://dx.doi.org/10.1021/jf60218a027 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/4204 |
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