Genetic Diversity of Pigeonpea (Cajanus cajan (L.) Millsp.) Cultivars and Its Wild Relatives Using Randomly Amplified Polymorphic DNA (RAPD) Markers

Yadav, K. and Yadav, S.K. and Yadav, A. and Pandey, V P and Dwivedi, U N (2012) Genetic Diversity of Pigeonpea (Cajanus cajan (L.) Millsp.) Cultivars and Its Wild Relatives Using Randomly Amplified Polymorphic DNA (RAPD) Markers. American Journal of Plant Sciences, 3 (3). pp. 322-330.

[img]
Preview
 PDF - Published Version
| Preview

Abstract

Genetic diversity among and between 16 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) and its wild relatives (C. albicans and C. lineatus) analysed using RAPD. Twenty two random primers with an average of 71.2% polymorphism produced 151 polymorphic bands. Cluster analysis based on these 151 RAPD markers revealed relatively low level (0.434 - 0.714) of genetic diversity among cultivars and high level of diversity between cultivars and wild relatives. C. albicans and C. lineatus showed only 0.231 similarity with each other and C. albicans showed relatively higher similarity with C. cajan cultivars than that showed by C. lineatus. In dendrogram the 16 cultivars grouped into two distinct clusters comprising of seven and nine genotypes each while the wild species form out groups. Bootstrap analysis of the dendrogram was performed and resulted in significant bootstrap values. Principal components analysis (PCA) also revealed the similar results that of unweighted pair group method with arithmetic mean (UPGMA). The first, second and third PCs contributed 55.9%, 5.9%, and 5.6% of the variation, respectively, with cumulative variation of the first three PCs was 67.4%

Item Type: Article
Additional Information: On the basis of results based on present study and previ-ous studies on pigeonpea we can conclude that there is an urgent need to incorporate diverse parents in breeding programmes of pigeonpea and generate more DNA mar- kers which can be useful in molecular breeding pro-grammes of this crop.
Uncontrolled Keywords: Genetic Diversity; RAPD; Pigeonpea
Author Affiliation: Department of Biochemistry, University of Lucknow, Lucknow, India;Department of Microbiology, College of Basic Science & Humanities, Sardarkrushinagar Dantiwada Agricultural University, Palanpur, India.
Subjects: Crop Improvement
Divisions: Pigeonpea
Depositing User: Mr. SanatKumar Behera
Date Deposited: 27 Mar 2012 04:48
Last Modified: 17 Apr 2012 10:30
Official URL: http://dx.doi.org/10.4236/ajps.2012.33038
URI: http://eprints.icrisat.ac.in/id/eprint/4201

Actions (login required)

View Item View Item
EPrints Logo
eprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.