Thidiazuron improves in vitro multiple shoot induction in chickpea (Cicer arietinum L.) cotyledon with embryonic axis

Pawar, B.D. and Jadhav, A.S. and Kale, A.A. and Chimote, V.P. and Pawar, S.V. (2012) Thidiazuron improves in vitro multiple shoot induction in chickpea (Cicer arietinum L.) cotyledon with embryonic axis. Journal of Food Legumes, 25 (1). pp. 9-13.

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Abstract

A reproducible and highly efficient plant regeneration method involving embryonic axis in chickpea Desi varieties, viz. ‘Vijay’, ‘Digvijay’ and Kabuli variety ‘Virat’ was developed as an essential pre-requisite for genetic transformation. Considerable variation in response to plant growth regulators used was observed in all the three cultivars. Embryonic axis with cotyledon obtained from 4–6 day old seedlings were found to be best in producing maximum of 55.02 shoot per explant when cultured on MSB5 medium with 2 mg/l 2-isopentenyladenine (2-iP), 1 mg/l thidiazuron (TDZ) and 0.4 mg/l kinetin (KIN). Embryonic axis without cotyledon produced only 2.25–4.88 shoots per explant. Shoots were elongated on MSB5 medium with 2 mg/l benzylaminopurine (BAP) and 0.1 mg/l indole acetic acid (IAA). Rooting was obtained by immersing cut ends of elongated shoots to 10 seconds pulse treatment of 100 mM indole butyric acid (IBA) and subsequent transfer to liquid MSB5 medium which resulted in maximum of 92.67% rooting. Plantlets with well-developed root systems were hardened in polyhouse out of which 72% survived and showed normal growth and development. This plant regeneration method was found to be genotype independent and deemed to be very useful in genetic transformation of chickpea.

Item Type: Article
Uncontrolled Keywords: Chickpea regeneration, Embryonic axis, Pulse treatment, Thidiazuron
Author Affiliation: State Level Biotechnology Centre, Mahatma Phule Krishi Vidyapeeth, Rahuri-413722, Maharashtra, India
Subjects: Crop Improvement
Divisions: Chickpea
Depositing User: Mr. SanatKumar Behera
Date Deposited: 24 Mar 2012 09:35
Last Modified: 24 Mar 2012 09:35
Official URL: http://www.indianjournals.com/ijor.aspx?target=ijo...
URI: http://eprints.icrisat.ac.in/id/eprint/4101

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