Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers

Amadou, H.I. and Bebeli, P.J. and Kaltsikes, P.J. (2001) Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers. Genome, 44 (6). pp. 995-999.

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Abstract

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.Key words: germplasm, PCR, RAPD, Vigna subterrane

Item Type: Article
Author Affiliation: Department of Plant Breeding and Biometry, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece
Subjects: Crop Improvement
Divisions: Groundnut
Depositing User: Mr. SanatKumar Behera
Date Deposited: 21 Mar 2012 07:54
Last Modified: 21 Mar 2012 07:54
Official URL: http://dx.doi.org/10.1139/g01-096
URI: http://eprints.icrisat.ac.in/id/eprint/4045

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