Pea (Pisum sativum L.) Protease Inhibitors from the Bowman-Birk Class Influence the Growth of Human Colorectal Adenocarcinoma HT29 Cells in Vitro

Clemente, A and Gee, J.M and Johnson, I.T. and et al, . (2005) Pea (Pisum sativum L.) Protease Inhibitors from the Bowman-Birk Class Influence the Growth of Human Colorectal Adenocarcinoma HT29 Cells in Vitro. Journal of Agriculture and Food Chemistry, 53. pp. 8979-8986.

[img] PDF - Published Version
Restricted to ICRISAT researchers only

Abstract

The Bowman-Birk trypsin-chymotrypsin inhibitor (BBI) from soybean has been described as a potential cancer chemopreventive agent. We have compared the effects of BBI with those of two variant recombinant pea (Pisum sativum L.) seed protease inhibitors, rTI1B and rTI2B, homologous to BBI but differing in inhibitory activity, on the growth of human colorectal adenocarcinoma HT29 cells in vitro. A significant and dose-dependent decrease in the growth of HT29 cells was observed using all protease inhibitors, with rTI1B showing the largest decrease (IC50 ) 46 íM). Inclusion of the pan-caspase inhibitor, Boc-D-FMK, did not negate the effects of rTI1B or rTI2B in the cell assays. The relative effectiveness of rTI1B and rTI2B may correlate with a variant amino acid sequence within their respective chymotrypsin inhibitory domain, in agreement with a chymotrypsin-like protease as a potential target.

Item Type: Article
Additional Information: Thanks are due to Dr. E. K. Lund (IFR, Norwich, U.K.) for her scientific advice. We thank Dr. G. Duc (INRA-URGAP, Dijon, France) for his gift of seeds of the pea genotypes D106 and D317. We are grateful to Professor A. M. Smith (JIC) for her constructive comments on this paper.
Uncontrolled Keywords: Bowman-Birk inhibitor; cell growth; chymotrypsin inhibitory domain; colon cancer cells; pea; Pisum sativum
Author Affiliation: Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH
Subjects: Plant Protection
Divisions: Other Crops
Depositing User: Mr Siva Shankar
Date Deposited: 07 Mar 2012 06:00
Last Modified: 07 Mar 2012 06:00
URI: http://eprints.icrisat.ac.in/id/eprint/3614

Actions (login required)

View Item View Item
EPrints Logo
eprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.