Minimum sample size and optimal positioning of flanking markers in marker-assisted backcrossing for transfer of a target gene

Frisch, M. and Bohn, M. and Melchinger, A.E. (1999) Minimum sample size and optimal positioning of flanking markers in marker-assisted backcrossing for transfer of a target gene. Crop Science, 39 (4). pp. 967-975.

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Abstract

In recurrent backcrossing designed for introgression of a target allele from a donor into the genetic background of a recurrent parent (RP), molecular markers can accelerate recovery of the recurrent parent genome (RPG). The objectives of this study were to determine in marker-assisted backcrossing (MAB) (i) the optimum distances (d1, d2) between the flanking markers and the target locus and (ii) the minimum number of individuals (n) required for obtaining with a certain probability a given number of individuals that carry the donor allele at the target locus and have a minimum proportion of donor genome on the carrier chromosome. Analytical solutions and tabulated results are given for relevant parameters (d1, d2, n) required to obtain, with a specified probability of success, at least one desired individual. They depend on the length of the carrier chromosome, the chromosomal position of the target locus, its distance to the flanking marker loci, and the number of individuals evaluated. The possibility of increasing the efficiency of MAB by reducing the number of individuals and marker data points required is discussed.

Item Type:	Article
Author Affiliation:	Institute of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim, 70593 Stuttgart, Germany
Subjects:	Crop Improvement > Genetics/Genomics Crop Improvement > Plant Breeding
Divisions:	Other Crops
Depositing User:	Syamala
Date Deposited:	01 Nov 2010 20:43
Last Modified:	29 Dec 2010 20:52
Official URL:	http://dx.doi.org/10.2135/cropsci1999.394967x
URI:	http://eprints.icrisat.ac.in/id/eprint/349

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