Yang, H and et al, . (1998) Transgenic peanut plants containing a nucleocapsid protein gene of tomato spotted wilt virus show divergent levels of gene expression. Plant Cell Reports , 17 (9). pp. 693-699.
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Abstract
The nucleocapsid protein (N) gene of the lettuce isolate of tomato spotted wilt virus (TSWV) was inserted into peanut (Arachis hypogaea L.) via microprojectile bombardment. Constructs containing the hph gene for resistance to the antibiotic hygromycin and the TSWV N gene were used for bombardment of peanut somatic embryos. High frequencies of transformation and regeneration of plants containing the N gene were obtained. Southern blot analysis of independent transgenic lines revealed that one to several copies of the N gene were integrated into the peanut genome. Northern blot, RT-PCR and ELISA analyses indicated that a gene silencing mechanism may be operating in primary transgenic lines containing multiple copy insertions of the N transgene. One transgenic plant which contained a single copy of the transgene expressed the N protein in the primary transformant, and the progeny segregated in a 3:1 ratio based upon ELISA determination
| Item Type: | Article |
|---|---|
| Additional Information: | Support for this work was provided by the Georgia Agricultural Commodity Commission for Peanuts and the National Peanut Foundation. We thank Dr. Hanu Pappu for assistance with ELISA and Anne Bell for technical assistance. |
| Author Affiliation: | Department of Horticulture, The University of Georgia Coastal Plain Experiment Station, Tifton, GA 31793, USA |
| Subjects: | Plant Production Plant Protection Crop Improvement |
| Divisions: | Groundnut |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 20 Feb 2012 14:07 |
| Last Modified: | 20 Feb 2012 14:08 |
| Official URL: | http://dx.doi.org/10.1007/s002990050467 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/3063 |
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