Tang , Y Y and et al, . (2011) Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.). Electronic Journal of Biotechnology, 14 (5). pp. 1-13.
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Abstract
To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).
| Item Type: | Article |
|---|---|
| Author Affiliation: | Ocean University of China, College of Marine Life Sciences, Qingdao, PR China |
| Subjects: | Crop Improvement |
| Divisions: | Groundnut |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 20 Feb 2012 13:02 |
| Last Modified: | 20 Feb 2012 13:02 |
| Official URL: | http://dx.doi.org/10.2225/vol14-issue5-fulltext-5 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/3060 |
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