Ramu, S.V. and Rohini, S. and Keshavareddy, G. and Gowri Neelima, M. and Shanmugam, N.B. and Kumar, R.V. and Sarangi, S.K. and Ananda Kumar, P. and Udayakumar, M. (2011) Expression of a synthetic cry1AcF gene in transgenic Pigeon pea confers resistance to Helicoverpa armigera. Journal of Applied Entomology. pp. 1-13.
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Abstract
Pigeon pea is an important legume. Yield losses due to insect pests are enormous in the cultivation of this crop. Expression of cry proteins has led to increased resistance to pests in several crops. We report in this paper, expression of a chimeric cry1AcF (encoding cry1Ac and cry1F domains) gene in transgenic pigeon pea and its resistance towards Helicoverpa armigera. PCR, Southern hybridization, RT-PCR and Western analysis confirmed stable integration and expression of the cry1AcF gene in pigeon pea transgenics. When screened for efficacy of the transformants for resistance against H. armigera, the transgenics showed not only high mortality of the larva but could also resist the damage caused by the larvae. Analysis for the stable integration, expression and efficacy of the transgenics resulted in the identification of four T3 plants arising from two T1 backgrounds as highly promising. The results demonstrate potentiality of the chimeric cry1AcF gene in developing H. armigera-resistant pigeon pea.
| Item Type: | Article |
|---|---|
| Additional Information: | Authors would like to thank Dr. Byregowda, Senior scientist (plant breeding) AICRP on pigeon pea, Uni- versity of Agricultural Sciences, GKVK, Bangalore for providing the seeds of pigeon pea and Dr. Jayanth, Pest control of India (PCI), Bangalore, India for providing insect larvae for the bioassays. |
| Uncontrolled Keywords: | Agrobacterium, genetic transformation, Helicoverpa armigera, insect resistance, inplanta transformation, pigeon pea |
| Author Affiliation: | Department of Microbiology and Biotechnology, Bangalore University, Bangalore, India |
| Subjects: | Plant Production Crop Improvement |
| Divisions: | Pigeonpea |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 13 Jan 2012 04:59 |
| Last Modified: | 13 Jan 2012 04:59 |
| Official URL: | http://onlinelibrary.wiley.com/journal/10.1111/(IS... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/2977 |
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