Molina, C. and Zaman-Allah, M. and Khan, F. and Fatnassi, N. and Horres, R. and Rotter, B. and Steinhauer, D. and Amenc, L. and Drevon, J-J. and Winter, P. and Kahl, G. (2011) The salt-responsive transcriptome of chickpea roots and nodules via deepSuperSAGE. BMC Plant Biology, 11. pp. 1-26.
![[img]](http://eprints.icrisat.ac.in/style/images/fileicons/application_pdf.png) |
PDF
- Published Version
Restricted to ICRISAT researchers only |
Abstract
The combination of high-throughput transcript profiling and next-generation sequencing technologies is a prerequisite for genome-wide comprehensive transcriptome analysis. Our recent innovation of deepSuperSAGE is based on an advanced SuperSAGE protocol and its combination with massively parallel pyrosequencing on Roche's 454 sequencing platform. As a demonstration of the power of this combination, we have chosen the salt stress transcriptomes of roots and nodules of the third most important legume crop chickpea (Cicer arietinum L.). While our report is more technology-oriented, it nevertheless addresses a major world-wide problem for crops generally: high salinity. Together with low temperatures and water stress, high salinity is responsible for crop losses of millions of tons of various legume (and other) crops. Continuously deteriorating environmental conditions will combine with salinity stress to further compromise crop yields. As a good example for such stress-exposed crop plants, we started to characterize salt stress responses of chickpeas on the transcriptome level.
| Item Type: | Article |
|---|---|
| Additional Information: | The authors thank Ruth Jüngling, University of Frankfurt, for technical advice, and the Array-On team for expert help with design of the microarrays. Work of the authors was supported by a grant from the Aquarhiz project (INCO- CT-2004-509115) to GK, grants from the Grain Legumes project (Food-CT- 2004-506223) to GK and GenXPro GmbH, and from DFG to GK (Ka 332/22-1) |
| Uncontrolled Keywords: | deepSuperSAGE, chickpea, SuperSAGE |
| Author Affiliation: | Johann Wolfgang Goethe University(Germany) |
| Subjects: | Plant Production Crop Improvement |
| Divisions: | Chickpea |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 05 Jan 2012 05:03 |
| Last Modified: | 05 Jan 2012 05:03 |
| Official URL: | http://www.biomedcentral.com/bmcplantbiol |
| URI: | http://eprints.icrisat.ac.in/id/eprint/2906 |
Actions (login required)
 |
View Item |
Altmetric
Altmetric