Davey, M W and Mellidou, I and Keulemans, W (2009) Considerations to prevent the breakdown and loss of fruit carotenoids during extraction and analysis in Musa. Journal of Chromatography A, 1216 (30). pp. 5759-5762.
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Abstract
The impact of treatments aimed at improving the robustness of protocols for the analysis of carotenoids in fruit of banana and plantain were examined. Neither the inclusion of polyvinylpolypyrrolidine in the extraction buffer, nor vigorous homogenisation with glass beads influenced recoveries or chromatographic profiles. By contrast, heating lead to losses of up to 53% and to the formation of degradation products that are no longer detectable on our RP-HPLC system. Carotenoid extracts are unstable and most sensitive to exposure to light. However, even in the dark at −20 °C and in the presence of antioxidants breakdown rates of around 5% per day were observed.
| Item Type: | Article |
|---|---|
| Additional Information: | The authors thank Otto van Poeselaere and Eva A. Davey forhelp in the preparation of the manuscript and Peter Salaets for technical help. The authors acknowledge financial support fromCGIAR-Bioversity and the Harvest Plus Global Challenge program on Nutritional Enhancement and Biodiversity, as well as Inge van den- Berg from Bioversity International, Montpellier, France and Gerard Bertin Ngoh-Newilah of CARBAP, Cameroon. |
| Uncontrolled Keywords: | Provitamin A carotenoids; Banana; Oxidation; Isomerisation; HPLC; Stability |
| Author Affiliation: | Katholieke Universiteit Leuven, De Croylaan 42, Heverlee, Leuven, Belgium |
| Subjects: | Plant Production Plant Protection |
| Divisions: | Other Crops |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 04 Jan 2012 10:10 |
| Last Modified: | 04 Jan 2012 10:10 |
| Official URL: | http://dx.doi.org/10.1016/j.chroma.2009.06.022 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/2889 |
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