A new set of allele-specific PCR primers for identification of true hybrids in normal oleate × high oleate crosses in groundnut

Wang, C T and Hu, D Q and Ding, F Y and Yu, T H and Tang , Y Y and Wang, Z X and Zhang, C (2011) A new set of allele-specific PCR primers for identification of true hybrids in normal oleate × high oleate crosses in groundnut. Journal of SAT Agricultural Research, 9. 4pp.

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Abstract

Allele-specific PCR (AS-PCR) with 3 primers was used to identify true F1 hybrids resulting from normal oleate groundnut cultivars pollinated with high oleate groundnut genotypes with a 1-bp insertion (A:T) at position 442 after the start codon in FAD2B. Its reliability was confirmed by direct sequencing of FAD2B PCR products. The AS-PCR protocol requires minimal resources, and thus provides an economical alternative to qPCR and CAPS methods.

Item Type: Article
Author Affiliation: Shandong Peanut Research Institute(SPRI), Qingdao
Subjects: Crop Improvement
Divisions: Groundnut
Depositing User: Mr Siva Shankar
Date Deposited: 05 Dec 2011 08:53
Last Modified: 05 Dec 2011 08:53
URI: http://eprints.icrisat.ac.in/id/eprint/2813

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