Pitt, J.I. and Hocking, A.D. and Glenn, D.R. (1983) An improved medium for the detection of Aspergillus flavus and A. parasiticus. Journal of Applied Bacteriology, 54 (1). pp. 109-114.
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Abstract
An effective selective medium for the enumeration of the 2 spp. was developed by modification of Bothast and Fennell's A. Differential Medium [RMVM 10, 1525]. Results can be obtained with the new medium, A. flavus and parasiticus Agar (AFPA), containing 20 g yeast extract, 10 g bacteriological peptone, 0.5 g ferric ammonium citrate, 0.1 g chloramphenicol, 1 ml dichloran sol. (0.2% in ethanol), 15 g agar, and distilled water to 1 l, after 42 h incubation at 30 deg C. The medium is thus suitable for use in quality control as a guide to the presence of A. flavus and, potentially, of aflatoxins. AFPA has been extensively tested on groundnuts and soils. Results were reproducible and comparable with those on standard fungal enumeration media incubated for much longer periods. A very low percentage of false positives or negatives was found
| Item Type: | Article |
|---|---|
| Author Affiliation: | CSIRO Div. Food Res., North Ryde, NSW 2133, Australia |
| Subjects: | Plant Protection |
| Divisions: | Groundnut |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 17 Nov 2011 09:20 |
| Last Modified: | 17 Nov 2011 09:20 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/2786 |
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