Senior, M.L. and Murphy, J.P. and Goodman, M.M. and Stuber, C.W. (1998) Utility of SSRs for determining genetic similarities and relationships in maize using an agarose gel system. Crop Science, 38 (4). pp. 1088-1098.
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Abstract
Some 94 elite maize inbred lines, representative of the genetic diversity among lines derived from the Corn Belt Dent and Southern Dent maize races, were assayed for polymorphism at 70 simple sequence repeats (SSR) marker loci using agarose gels. The 365 alleles identified served as raw data for estimating genetic similarities among these lines. The patterns of genetic divergence revealed by the SSR polymorphisms were consistent with known pedigrees. A cluster analysis placed the inbred lines in clusters that correspond to major heterotic groups or market classes for North American maize. A unique fingerprint for each inbred line could be obtained from as few as 5 SSR loci. The utility of PCR-based markers such as SSRs for measuring genetic diversity, for assigning lines to heterotic groups and for genetic fingerprinting equals or exceeds that of RFLP markers, a property that may prove a valuable asset for a maize breeding programme.
| Item Type: | Article |
|---|---|
| Author Affiliation: | USDA-ARS, Molecular Marker Facility, Box 7620, North Carolina State University, Raleigh, NC 27695, USA |
| Subjects: | Crop Improvement > Genetics/Genomics Crop Improvement > Plant Breeding Crop Improvement > Biotechnology |
| Divisions: | Maize |
| Depositing User: | Sandhya Gir |
| Date Deposited: | 15 Oct 2010 03:24 |
| Last Modified: | 15 Oct 2010 03:24 |
| Official URL: | https://www.crops.org/publications/cs/abstracts/38... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/27 |
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