Chickpea Hybridization Using In Vitro Techniques

Mallikarjuna, N. and Muehlbauer, F.J. (2011) Chickpea Hybridization Using In Vitro Techniques. In: Plant Embryo Culture Methods and Protocols. Methods in Molecular Biology, 710 (Part 2). Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA, School of Life Sciences, University of Hertfordshire, Hartfield, Hertfordshire, AL10 9AB, UK, pp. 93-105. ISBN ISBN 978-1-61737-988-8

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Abstract

Tissue culture techniques play an important role in the utilization of wild Cicer species for the improvement of cultivated chickpea. Utilization of wild Cicer species has become essential as a series of evolutionary bottlenecks have narrowed the genetic base of chickpea, thus making it susceptible to a range of diseases and pests. Crosses with wild Cicer can broaden its genetic base and introduce useful traits. Except for two wild species, none of the other Cicer species are cross-compatible. To use a range of Cicer species for the improvement of chickpea, embryo rescue and tissue culture techniques are necessary. The success of the cross with incompatible species depended on a range of techniques including the application of growth regulators to pollinated pistils and saving aborting embryos in vitro. Further, the chances of successful transfer of hybrid shoots to soil are greater if the hybrid shoots are grafted to chickpea stocks

Item Type:	Book Section
Uncontrolled Keywords:	Chickpea, Cicer species, Cleft graft, Embryo rescue, Growth regulators, Incompatibility
Author Affiliation:	Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada
Subjects:	Crop Improvement
Divisions:	Chickpea
Depositing User:	Ms K Syamalamba
Date Deposited:	16 Aug 2011 08:00
Last Modified:	16 Aug 2011 08:00
Official URL:	http://dx.doi.org/10.1007/978-1-61737-988-8_8
URI:	http://eprints.icrisat.ac.in/id/eprint/2570

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