Identification and characterisation of sugarcane intergeneric hybrids, Saccharum officinarum x Erianthus arundinaceus, with molecular markers and DNA in situ hybridisation

D'Hont, A. and Rao, P.S. and Feldmann, P. and Grivet, L. and Islam-Faridi, N. and Taylor, P. and Glaszmann, J.C. (1995) Identification and characterisation of sugarcane intergeneric hybrids, Saccharum officinarum x Erianthus arundinaceus, with molecular markers and DNA in situ hybridisation. TAG Theoretical and Applied Genetics, 91 (2). pp. 320-326.

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Abstract

Molecular markers were used to characterise sugarcane intergeneric hybrids between S. officinarum and E. arundinaceus. Very simple diagnostic tools for hybrid identification among the progeny were derived from isozyme electrophoresis and a sequence-tagged PCR. Two enzyme systems (GOT and MDH B) and PCR amplification revealing spacer-size variation in the 5s-rDNA cluster were found most convenient. Specific characterisation of the two genomic components was possible using RFLP and in situ hybridisation. The strong molecular differentiation between S. officinarum and E. arundinaceus allows the identification of numerous Erianthus-specific RFLP bands in the hybrids. Genomic DNA in situ hybridisation allows for the differentiation of the chromosomes contributed by S. officinarum and E. arundinaceus in chromosome preparations of the hybrids. In situ hybridisation with the 18s-5.8s-25s rDNA probe highlights the basic chromosome numbers in the two parental species. The potential of these techniques to monitor the Erianthus genome during the introgression process is discussed.

Item Type: Article
Author Affiliation: CIRAD, BP 5035, 34032 Montpellier, France
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Divisions: Other Crops
Depositing User: Mr T L Gautham
Date Deposited: 22 Oct 2010 07:41
Last Modified: 09 Dec 2010 22:59
Official URL: http://dx.doi.org/10.1007/BF00220894
URI: http://eprints.icrisat.ac.in/id/eprint/257

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