Carroll, J. and Li, J. and Ellar, D.J. (1989) Proteolytic processing of a coleopteran-specific delta-endotoxin produced by Bacillus thuringiensis var. tenebrionis. Biochemical Journal, 261 (1). pp. 99-105.
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Abstract
Insecticidal protein delta-endotoxin crystals harvested from sporulated cultures of Bacillus thuringiensis var. tenebrionis contain a major polypeptide of 67 kDa and minor polypeptides of 73, 72, 55 and 46 kDa. During sporulation, only the 73 kDa polypeptide could be detected at stage I. The 67 kDa polypeptide was first detected at stage II and increased in concentration throughout the later stages of sporulation and after crystal release, with a concomitant decrease in the 73 kDa polypeptide. This change could be blocked by the addition of proteinase inhibitors. Trypsin or insect-gut-extract treatment of the delta-endotoxin crystals after solubilization resulted in a cleavage product of 55 kDa with asparagine-159 of the deduced amino acid sequence of the toxin [Höfte, Seurinck, van Houtven & Vaeck (1987) Nucleic Acids Res. 15, 71-83; Sekar, Thompson, Maroney, Bookland & Adang (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 7036-7040; McPherson, Perlak, Fuchs, Marrone, Lavrik & Fischhoff (1988) Biotechnology 6, 61-66] at the N-terminus. This polypeptide was found to be as toxic in vivo as native delta-endotoxin
| Item Type: | Article |
|---|---|
| Author Affiliation: | Department of Biochemistry, University of Cambridge, U.K |
| Subjects: | Plant Protection > Pesticides Plant Protection > Pests |
| Divisions: | Other Crops |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 01 Aug 2011 08:58 |
| Last Modified: | 01 Aug 2011 08:58 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/2503 |
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