Gajula, C. and Chandel, A.K. and Konakalla, R. and Rudravaram, R. and Pogaku, R. and Mangamoori, L.N. (2011) Fermentation of groundnut shell enzymatic hydrolysate for fuel ethanol production by free and sorghum stalks immobilized cells of pichia stipitis NCIM 3498. International Journal of Chemical Reactor Engineering, 9 (A61). 19pp.
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Abstract
Groundnut shell (GS), after separation of pod, is readily available as a potential feedstock for production of fermentable sugars. The substrate was delignified with sodium sulfite. The delignified substrate released 670 mg/g of sugars after enzymatic hydrolysis (50°C, 120 rpm, 50 hrs) using commercial cellulases (Dyadic® Xylanase PLUS, Dyadic Inc. USA). The groundnut shell enzymatic hydrolysate (45.6 g/L reducing sugars) was fermented for ethanol production with free and sorghum stalks immobilized cells of Pichia stipitis NCIM 3498 under submerged cultivation conditions. Immobilization of yeast cells on sorghum stalks were confirmed by scanning electron microscopy (SEM). A maximum of ethanol production (17.83 g/L, yield 0.44 g/g and 20.45 g/L, yield 0.47 g/g) was observed with free and immobilized cells of P. stipitis respectively in batch fermentation conditions. Recycling of immobilized cells showed a stable ethanol production (20.45 g/L, yield 0.47 g/g) up to 5 batches followed by a gradual downfall in subsequent cycles.
| Item Type: | Article |
|---|---|
| Author Affiliation: | Jawaharlal Nehru Technological University |
| Subjects: | Plant Production Agricultural Engineering |
| Divisions: | Groundnut |
| Depositing User: | Sandhya Gir |
| Date Deposited: | 25 Jul 2011 05:38 |
| Last Modified: | 29 Dec 2011 03:01 |
| Official URL: | http://www.bepress.com/ijcre/vol9/A61/ |
| URI: | http://eprints.icrisat.ac.in/id/eprint/2454 |
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