Horsch, R.B. and Fry, J.E. and Hoffmann, N.L. and Eichholtz, D. and Rogers, S.G. and Fraley, R.T. (1985) A simple and general method for transferring genes into plants. Science, 227 (4691). pp. 1229-1231.
![[img]](http://eprints.icrisat.ac.in/style/images/fileicons/application_pdf.png) |
PDF
Restricted to ICRISAT researchers only |
Abstract
Surface-sterilized leaf discs from petunia, tobacco and tomato were inoculated with an Agrobacterium tumefaciens strain containing a modified Ti plasmid with a chimaeric gene for kanamycin resistance. The discs were cultured for 2 days and then transferred to a selection medium containing kanamycin. Shoot regeneration occurred within 2 to 4 weeks, and transformants were confirmed by their ability to form roots in medium containing kanamycin. This method thus combines gene transfer, plant regeneration and selection for transformants in a single step
| Item Type: | Article |
|---|---|
| Author Affiliation: | Biological Sciences, Monsanto Company, St. Louis, Missouri 63167, USA. |
| Subjects: | Crop Improvement > Genetics/Genomics |
| Divisions: | General |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 25 Jul 2011 04:34 |
| Last Modified: | 25 Jul 2011 04:34 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/2448 |
Actions (login required)
 |
View Item |
