Murai, N. and Sutton, D.W. and Murray, M.G. and Slightom, J.L. and Merlo, D.J. and et al, . (1983) Phaseolin gene from bean is expressed after transfer to sunflower via tumor-inducing plasmid vectors. Science, 222 (4623). pp. 476-482.
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Abstract
Ti plasmids were constructed which contained Phaseolus vulgaris DNA coding for phaseollin under the transcriptional control of its own promoter or the octopine synthase promoter. Sunflower stem tissues in situ (seedlings) and in vitro were inoculated with Agrobacterium tumefaciens containing these plasmids. The RNA transcribed in transformed cells was characterized by hybridization procedures, S1 nuclease mapping and in vitro translation. Results showed that genomic intervening sequences had been correctly excised. Immunoreactive phaseollin polypeptides were detected by enzyme-linked immunosorbent assay and antibody hybridization to electrophoretically separated protein extracts of tissues isolated from crown gall tumours which developed on the seedlings and from the transformed cells grown in tissue culture
| Item Type: | Article |
|---|---|
| Author Affiliation: | Department of Plant Pathology, University Wisconsin, Madison 53706, USA |
| Subjects: | Crop Improvement > Genetics/Genomics Crop Improvement > Plant Breeding |
| Divisions: | Other Crops |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 25 Jul 2011 04:34 |
| Last Modified: | 25 Jul 2011 04:34 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/2447 |
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