Klein, T.M. and Wolf, E.D. and Wu, R. and Sanford, J.C. (1987) High-velocity microprojectiles for delivering nucleic acids into living cells. Nature, 327 (6117). pp. 70-73.
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Abstract
A method is described in which DNA or RNA is delivered into plant cells using small tungsten particles (microprojectiles; 4 µm diameter) at high velocity. After acceleration, the particles pierce the cell walls and membranes and enter the intact cells without killing them. Using this method, 1 cm² sections of Allium cepa epidermis were bombarded with tungsten particles coated with plasmid p35S-CAT, containing a gene that encodes chloramphenicol acetyltransferase (CAT). Extracts subsequently obtained from the tissue revealed expression of the gene, in the form of very high levels of CAT activity; controls showed negligible activity. It is suggested that the method can be used to study the transient expression of foreign genes in intact tissue and can possibly provide a broadly applicable means of transformation capable of circumventing the host range restrictions of Agrobacterium tumefaciens and the regeneration problems associated with protoplasts
| Item Type: | Article |
|---|---|
| Author Affiliation: | Department of Horticultural Science, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456, USA |
| Subjects: | Crop Improvement > Genetics/Genomics Crop Improvement > Plant Breeding |
| Divisions: | General |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 25 Jul 2011 04:35 |
| Last Modified: | 25 Jul 2011 04:35 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/2446 |
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