The biolistic® PDS-1000/he device

Kikkert, J.R. (1993) The biolistic® PDS-1000/he device. Plant Cell Tissue and Organ Culture, 33 (3). pp. 221-226.

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Abstract

This paper describes the design, operation, and performance of the Biolistic® PDS-1000/He device, which is used to transform living organisms with foreign DNA. DNA is delivered to cells in association with microscopic metal particles, called microcarriers, that are propelled at high velocity towards target tissues. The microcarriers are accelerated on a plastic cylinder, called a macrocarrier, which is driven by a shock wave of helium gas. The effectiveness of the PDS-1000/He device was tested by bombarding tobacco cell suspension cultures with microcarriers that were coated with plasmid DNA containing the B-glucuronidase (GUS) and neomycin phosphotransferase II (NPTII) genes. Two days after bombardment, there were 6835 ± 594 cell clusters per petri plate that expressed the GUS gene. Kanamycin resistant colonies were observed 6 to 8 weeks after bombardment, at a rate of 838 ± 134 colonies per bombarded plate

Item Type: Article
Author Affiliation: Carnell University, New York State Agricultural Experiment Station, Department of Horticultural Sciences, Geneva, NY 14456, USA
Subjects: Crop Improvement
Divisions: General
Depositing User: Mr B Krishnamurthy
Date Deposited: 25 Jul 2011 04:40
Last Modified: 25 Jul 2011 04:40
URI: http://eprints.icrisat.ac.in/id/eprint/2441

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