Insect tolerant transgenic tomato plants

Fischhoff, D.A. and Bowdish, K.S. and Perlak, F.J. and Marrone, P.G. and McCormick, S.M. and et al, . (1987) Insect tolerant transgenic tomato plants. Bio/Technology, 5 (8). pp. 807-813.

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Abstract

The structure of an insect control protein gene from Bacillus thuringiensis subsp. kurstaki strain HD1 was determined and truncated forms of the gene were generated. Two of these truncated genes were incorporated into vectors and, via Agrobacterium-mediated transformation, were introduced singly into tomato plants. The chimaeric genes were expressed in the transgenic plants, conferring tolerance to lepidopteran larvae. Larvae of Manduca sexta, Heliothis zea [Helicoverpa zea] and Heliothis virescens exhibited up to 100% mortality when fed on leaves of the transformed plants. The introduced gene was stably transmitted to the plants' progeny, segregating as a single dominant Mendelian factor

Item Type: Article
Author Affiliation: Monsanto Co., 700 Chesterfield Village Parkway, St. Louis, MO 63198, USA
Subjects: Plant Protection > Pesticides
Plant Protection > Pests
Divisions: Other Crops
Depositing User: Mr B Krishnamurthy
Date Deposited: 25 Jul 2011 04:44
Last Modified: 25 Jul 2011 04:44
Official URL: http://dx.doi.org/10.1038/nbt0887-807
URI: http://eprints.icrisat.ac.in/id/eprint/2438

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