Generation of sweet orange transgenic lines and evaluation of citrus psorosis virus-derived resistance against psorosis A and psorosis B

Reyes, C.A. and Zanek, M.C. and Velázquez, K. and Costa, N. and Plata, M.I. and García, M.L. (2011) Generation of sweet orange transgenic lines and evaluation of citrus psorosis virus-derived resistance against psorosis A and psorosis B. Journal of Phytopathology, 159 (7-8). pp. 531-537.

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Abstract

Citrus psorosis is a widespread serious disease of citrus caused by Citrus psorosis virus (CPsV). In Argentina and Uruguay, this disease is spread by an unknown vector and there is no natural resistance or tolerance to the disease. There are two types of psorosis, described according to the symptoms observed in cit- rus trees, psorosis A (PsA) and psorosis B (PsB). PsA protects against the severe effects of the more aggres- sive type PsB. We have applied pathogen-derived resis- tance to create a defence mechanism against this virus disease. Sweet orange transgenic lines were obtained containing three different genes of CPsV (54k, 48k and 24k genes) taken from a PsA isolate (CPV-4). Four- teen lines were selected containing 1, 2 or 3 copies of the transgenes and evaluated for their acquired resis- tance against PsA (CPV 4 from USA) and PsB (CPsV 189-34 from Argentina) isolates. These lines were sus- ceptible to both isolates when graft-infected, although one of the lines carrying the cp gene (CP-96 line) con- taining two copies of the transgene and expressing a low level of the coat protein showed a delay in symp- tom expression when inoculated with the PsB isolate.

Item Type: Article
Uncontrolled Keywords: Citrus psorosis virus; citrus transgenic plants; Ophiovirus; psorosis A; psorosis B; pathogen-derived resistance;Argentina
Author Affiliation: Instituto de Biotecnología y Biología Molecular, CCT-La Plata, CONICET – UNLP, La Plata, Buenos Aires, Argentina
Subjects: Plant Production
Plant Protection
Divisions: Other Crops
Depositing User: Sandhya Gir
Date Deposited: 18 Jul 2011 04:03
Last Modified: 18 Jul 2011 04:05
Official URL: http://dx.doi.org/10.1111/j.1439-0434.2011.01800.x
URI: http://eprints.icrisat.ac.in/id/eprint/2394

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