Lenka, S.K. and Lohia, B. and Kumar, A. and Chinnusamy, V. (2009) Genome-wide targeted prediction of ABA responsive genes in rice based on over-represented cis-motif in co-expressed genes. Plant Molecular Biology, 69 (3). pp. 261-271.
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Abstract
Abscisic acid (ABA), the popular plant stress hormone, plays a key role in regulation of sub-set of stress responsive genes. These genes respond to ABA through specific transcription factors which bind to cis-regulatory elements present in their promoters. We discovered the ABA Responsive Element (ABRE) core (ACGT) containing CGMCACGTGB motif as over-represented motif among the promoters of ABA responsive co-expressed genes in rice. Targeted gene prediction strategy using this motif led to the identification of 402 protein coding genes potentially regulated by ABA-dependent molecular genetic network. RT-PCR analysis of arbitrarily chosen 45 genes from the predicted 402 genes confirmed 80% accuracy of our prediction. Plant Gene Ontology (GO) analysis of ABA responsive genes showed enrichment of signal transduction and stress related genes among diverse functional categories
| Item Type: | Article |
|---|---|
| Author Affiliation: | National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi 110 012, India |
| Subjects: | Crop Improvement Crop Improvement > Genetics/Genomics |
| Divisions: | Other Crops |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 14 Jul 2011 07:58 |
| Last Modified: | 14 Jul 2011 07:58 |
| Official URL: | http://dx.doi.org/10.1007/s11103-008-9423-4 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/2360 |
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