Isolated microspore culture of chickpea (Cicer arietinum L.): induction of androgenesis and cytological analysis of early haploid divisions

Croser, J.S. and Lulsdorf, M.M. and Grewal, R.K. and Usher, K.M. and Siddique, K.H.M. (2011) Isolated microspore culture of chickpea (Cicer arietinum L.): induction of androgenesis and cytological analysis of early haploid divisions. In Vitro Cellular and Developmental Biology - Plant, 47 (3). pp. 357-368.

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Abstract

Routine production of haploid plants has not been reported for any legume, despite the major role these species play in sustainable farming systems and human nutrition. It is within this context that we report a protocol for the induction of haploid development in chickpea (Cicer arietinum L.) using isolated microspore culture. The cultivars “Rupali”, “Narayen”, and “Kimberley Large” were identified as responsive to isolated microspore culture. Flower bud length and microspore developmental stage were correlated for these cultivars. Depending on the cultivar, buds 2.85–3.5 mm in length contained uninucleate microspores. Microspores from donor plants grown in winter and spring were more responsive than those grown in summer. A cold treatment (4°C) of between 24 and 48 h enhanced microspore response in winter- and spring-grown material but was not effective in summer-grown material. A medium developed by the authors was effective for microspore induction and early-stage embryo development. The addition of hormones to this medium was promotive of microspore induction in winter- and spring-grown material, but not in summer-grown material. The initial haploid division predominantly occurred via symmetrical division of the vegetative nucleus. Further research is under way to convert pro-embryos into plants

Item Type: Article
Author Affiliation: Centre for Legumes in Mediterranean Agriculture, The University of Western Australia, 35 Stirling Hwy, Crawley, WA, Australia 6009
Subjects: Plant Production
Divisions: Chickpea
Depositing User: Syamala
Date Deposited: 24 Jun 2011 09:28
Last Modified: 24 Jun 2011 09:28
Official URL: http://dx.doi.org/10.1007/s11627-011-9346-7
URI: http://eprints.icrisat.ac.in/id/eprint/2291

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