Telomere length dynamics in pearl millet (Pennisetum glaucum [L.] R. Br.) as observed at different developmental stages

Devi, K.U. and Devi, V.S. and Nirmala, K. and Sivaramakrishnan, S. and Isola, N.R. (2003) Telomere length dynamics in pearl millet (Pennisetum glaucum [L.] R. Br.) as observed at different developmental stages. Plant Biology, 5 (3). 285 -289.

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Abstract

To search for the pattern(s) operating in plants, a study of the telomere length was made in pearl millet. Telomere length in cells representing different developmental stages: (a) embryo, (b) leaves of one-week-old seedlings, one-month-old plants and boot leaf and (c) germ cells (pollen) were compared. The presence of the consensus plant telomere repeat sequence (5′-TTTAGGG-3′) in pearl millet telomeres was first ascertained; the sensitivity of the sequences hybridizing with (TTTAGGG)4 oligonucleotide to time course Bal 31 exonuclease digestions were studied on dot blots and gel blots. The exonuclease digestion kinetics revealed the presence of the consensus telomere repeat sequence in pearl millet telomeres. The average telomere length (ATL) was measured from autoradiograms of Hae III digested DNA, hybridized with labelled (5′-TTTAGGG-3′)4 oligonucleotide using UVI band software. No significant difference in the average telomere length was observed between the embryo, leaves of one-week-old seedlings, boot leaf and pollen. The ATL in leaves of one-month-old plants was slightly higher than ATLs in other crop growth stages. The results of the present investigation and analysis of the reports in the other plants suggest that there is an occasional increase in telomere length in some telomeres but no significant decrease due to loss during DNA replication

Item Type: Article
Author Affiliation: Department of Botany, Andhra University, Visakhapatnam, 530 003, India
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Divisions: Millet
Depositing User: Syamala
Date Deposited: 14 Jun 2011 05:12
Last Modified: 14 Jun 2011 05:12
Official URL: http://dx.doi.org/10.1055/s-2003-40800
URI: http://eprints.icrisat.ac.in/id/eprint/2179

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