Jing, Tan and Baisakh, Niranjan and Oliva, N. and Parkhi, Vilas and Rai, Mayank and Torrizo, L. and Datta, Karabi and Datta, S.K. (2005) The screening of rice germplasm, including those transgenic rice lines which accumulate β-carotene in their polished seeds, for their carotenoid profile. International Journal of Food Science & Technology, 40 (5). pp. 563-569.
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Abstract
A large number of exotic and commercial rice cultivars were screened to explore the carotenoid profile of the rice germ plasm. The data were compared with that of some transgenic plants where the genes for the entire biochemical pathway used to synthesize β-carotene had been engineered into the plant in such a way that it was hypothesized that β-carotene would be detectable in polished seeds, when normally polished seeds do not contain β-carotene. Eighty-eight rice accessions were used for the screening; 79 were obtained from the germ plasm bank of the International Rice Research Institute in the Philippines. Nine cultivars (Basmati 370, BR28, BR 29, BR-R, Kranti, Limpopo, Mahsuri, NDR359 and Pantdhan4) from other countries were also included. Among the 88 non-transgenic rice accessions (including BR-R, an elite restorer line), 69 cultivars were screened, using both polished ad unpolished samples and 19 cultivars were screened for unpolished seeds only. Forty-nine cultivars (unpolished) were analysed using the 85-min HPLC system, whereas 40 accessions (polished and unpolished) were run using the 30 min HPLC method. None of the polished cultivars were identified as containing β-carotene when both HPLC conditions were used. When unpolished, a majority of the rice accessions showed both lutein and β-carotene. Chlorophyll and other unidentified peaks were eluted between lutein and β-carotene in immature seeds. Transgenic rice contained not only β-carotene and lutein but also other carotenoids after polishing.
| Item Type: | Article |
|---|---|
| Author Affiliation: | Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines |
| Subjects: | Postharvest Management > Food Technology Crop Improvement > Genetics/Genomics |
| Divisions: | Other Crops |
| Depositing User: | Sandhya Gir |
| Date Deposited: | 21 Oct 2010 05:48 |
| Last Modified: | 21 Oct 2010 10:41 |
| Official URL: | http://dx.doi.org/10.1111/j.1365-2621.2005.00971.x |
| URI: | http://eprints.icrisat.ac.in/id/eprint/215 |
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