Paine, J.A. and Shipton, C.A. and Chaggar, Sunandha and Howells, R.M. and Kennedy, M.J. and Vernon, G. and Wright, S.Y. and Hinchliffe, E. and Adams, J.L. and Silverstone, A.L. and Drake, R. (2005) Improving the nutritional value of Golden Rice through increased pro-vitamin A content. Nature Biotechnology, 23 (4). pp. 482-487.
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Abstract
'Golden Rice' is a variety of rice engineered to produce β-carotene (pro-vitamin A) to help combat vitamin A deficiency, and it has been predicted that its contribution to alleviating vitamin A deficiency would be substantially improved through even higher β-carotene content. We hypothesized that the daffodil gene encoding phytoene synthase (psy), one of the two genes used to develop Golden Rice, was the limiting step in β-carotene accumulation. Through systematic testing of other plant psys, we identified a psy from maize that substantially increased carotenoid accumulation in a model plant system. We went on to develop 'Golden Rice 2' introducing this psy in combination with the Erwinia uredovora [Pantoea ananatis pv. uredovora] carotene desaturase (crtI) used to generate the original Golden Rice. We observed an increase in total carotenoids of up to 23-fold (maximum 37 µg/g) compared to the original Golden Rice and a preferential accumulation of β-carotene.
| Item Type: | Article |
|---|---|
| Author Affiliation: | Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire, RG42 6EY, UK. |
| Subjects: | Crop Improvement > Genetics/Genomics Crop Improvement > Plant Breeding Crop Improvement > Biotechnology |
| Divisions: | Other Crops |
| Depositing User: | Sandhya Gir |
| Date Deposited: | 21 Oct 2010 05:09 |
| Last Modified: | 21 Oct 2010 05:09 |
| Official URL: | http://www.nature.com/nbt/ |
| URI: | http://eprints.icrisat.ac.in/id/eprint/210 |
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