Evaluation of in vitro responses from different explants of elite Jatropha curcas L.

Verma, K.C. and Gaur, A.K. and Singh, U.S. (2008) Evaluation of in vitro responses from different explants of elite Jatropha curcas L. Indian Journal of Plant Physiology, 13 (3). 231-237 .

[img] PDF
Restricted to ICRISAT researchers only

Abstract

A regeneration protocol was optimized for the faster propagation of elite Jatropha curcas, an important biodiesel plant. Out of different explants tested (petiole, apical bud and leaf), apical buds were found to be the best for callus induction. Shoot regeneration from calluses induced from petioles was the best. Various combinations of auxins with cytokinins were suitable for callus induction. The best shoot regeneration (75%) was observed in MS medium supplemented with IBA (1.23 µM) and BAP [benzyladenine] (6.6 µM). Root induction (100%) was successfully obtained in MS and 1/2 MS medium. Acclimatization and hardening was quite successful with survival rate of 75%.

Item Type: Article
Author Affiliation: Department of Biochemistry, College of Basic Sciences and Humanities, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar - 263 145, Uttarakhand, India
Subjects: Plant Physiology and Biochemistry > Plant Physiology
Divisions: Other Crops
Depositing User: Sandhya Gir
Date Deposited: 25 May 2011 05:49
Last Modified: 25 May 2011 05:49
URI: http://eprints.icrisat.ac.in/id/eprint/2071

Actions (login required)

View Item View Item