Ozsolak, F. and Milos, P.M. (2011) RNA sequencing: advances, challenges and opportunities. Nature Reviews Genetics, 12 (2). pp. 87-98.
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Abstract
In the few years since its initial application, massively parallel cDNA sequencing, or RNA-seq, has allowed many advances in the characterization and quantification of transcriptomes. Recently, several developments in RNA-seq methods have provided an even more complete characterization of RNA transcripts. These developments include improvements in transcription start site mapping, strand-specific measurements, gene fusion detection, small RNA characterization and detection of alternative splicing events. Ongoing developments promise further advances in the application of RNA-seq, particularly direct RNA sequencing and approaches that allow RNA quantification from very small amounts of cellular materials
| Item Type: | Article |
|---|---|
| Author Affiliation: | Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA |
| Subjects: | Crop Improvement > Genetics/Genomics |
| Divisions: | General |
| Depositing User: | Syamala |
| Date Deposited: | 11 May 2011 04:59 |
| Last Modified: | 11 May 2011 04:59 |
| Official URL: | http://dx.doi.org/10.1038/nrg2934 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/2014 |
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