Metzker, M.L. (2010) Sequencing technologies — the next generation. Nature Reviews Genetics, 11 (1). pp. 31-46.
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Abstract
Demand has never been greater for revolutionary technologies that deliver fast, inexpensive and accurate genome information. This challenge has catalysed the development of next-generation sequencing (NGS) technologies. The inexpensive production of large volumes of sequence data is the primary advantage over conventional methods. Here, I present a technical review of template preparation, sequencing and imaging, genome alignment and assembly approaches, and recent advances in current and near-term commercially available NGS instruments. I also outline the broad range of applications for NGS technologies, in addition to providing guidelines for platform selection to address biological questions of interest
| Item Type: | Article |
|---|---|
| Author Affiliation: | Human Genome Sequencing Center and Department of Molecular & Human Genetics, Baylor College of Medicine, One Baylor Plaza, N1409, Houston, Texas 77030, USA |
| Subjects: | Plant Protection > Control Methods Environmental Science > Natural Resources |
| Divisions: | General |
| Depositing User: | Syamala |
| Date Deposited: | 11 May 2011 05:02 |
| Last Modified: | 11 May 2011 05:02 |
| Official URL: | http://dx.doi.org/10.1038/nrg2626 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/2009 |
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